Intercoder Reliability Assessment of Supplemental Document Coding in a Quantitative Content Analysis Study of Police Crime in the United States

Dr. Stinson maintains an object-relational research database in OnBase, Bowling Green State University’s enterprise-level content management system. An analysis of the intercoder reliability of the supplemental articles indexed in OnBase during the years 2012-2014 was conducted to determine and promote reliability among research assistants. This project is important because reliability is the hallmark of any research database, and because the institutional memory is short with annual turnover of student research assistants working in Stinson’s research group. Training recommendations are made from the findings of this study for the purpose of improving the reliability of document coding in the project object database

Parental safety concerns and active school commute: correlates across multiple domains in the home-to-school journey

Abiodun O. Oluyomi, Eileen Nehme, and Deanna M. Hoelscher are with The Michael and Susan Dell Center for Healthy Living, UT School of Public Health, Austin Regional Campus, The University of Texas Health Science Center at Houston, Austin, TX USA -- Chanam Lee is with the Department of Landscape Architecture and Urban Planning, College of Architecture, Texas AM University, College Station, TX USA -- Diane Dowdy and Marci G. Ory are with the School of Rural Public Health, Texas AM HeaBackground: Empirical evidence of the relationship between safety concerns and walking to school (WTS) is growing. However, current research offers limited understanding of the multiple domains of parental safety concerns and the specific mechanisms through which parents articulate safety concerns about WTS. A more detailed understanding is needed to inform environmental and policy interventions. This study examined the relationships between both traffic safety and personal safety concerns and WTS in the U.S. Methods: This cross-sectional analysis examined data from the Texas Childhood Obesity Prevention Policy Evaluation (T-COPPE) project, an evaluation of state-wide obesity prevention policy interventions. All study data were from the survey (n = 830) of parents with 4th grade students attending 81 elementary schools across Texas, and living within two miles from their children's schools. Traffic safety and personal safety concerns were captured for the home neighborhood, en-route to school, and school environments. Binary logistic regression analysis was used to assess the odds of WTS controlling for significant covariates. Results: Overall, 18% of parents reported that their child walked to school on most days of the week. For traffic safety, students were more likely to walk to school if their parent reported favorable perceptions about the following items in the home neighborhood environment: higher sidewalk availability, well maintained sidewalks and safe road crossings. For the route to school, the odds of WTS were higher for those who reported "no problem" with each one of the following: traffic speed, amount of traffic, sidewalks/pathways, intersection/crossing safety, and crossing guards, when compared to those that reported "always a problem". For personal safety in the en-route to school environment, the odds of WTS were lower when parents reported concerns about: stray or dangerous animals and availability of others with whom to walk. Conclusions: Findings offered insights into the specific issues that drive safety concerns for elementary school children’s WTS behaviors. The observed associations between more favorable perceptions of safety and WTS provide further justification for practical intervention strategies to reduce WTS barriers that can potentially bring long-term physical activity and health benefits to school-aged children.Public [email protected]

The assessment in the Primary Education classroom. Key factor for the learning science and mathematics

En este artículo se presenta una experiencia desarrollada en un aula de 6º de primaria. Después de una breve justificación de la perspectiva desde la que se fueron tomando las decisiones presentamos la propuesta desarrollada en torno a un proceso de evaluación. Dicho proceso está basado en el seguimiento del reconocimiento y superación del error por parte de los alumnos en relación con el conocimiento de las facciones y su combinación, como parte fundamental para entender aspectos relacionados con la proporcionalidad

<i>Phaeocystis globosa</i> Virus DNA Polymerase X: a “Swiss Army knife”, Multifunctional DNA polymerase-lyase-ligase for Base Excision Repair

Phaeocystis globosa virus 16T is a giant virus that belongs to the so-called nucleo-cytoplasmic large DNA virus (NCLDV) group. Its linear dsDNA genome contains an almost full complement of genes required to participate in viral base excision repair (BER). Among them is a gene coding for a bimodular protein consisting of an N-terminal Polβ-like core fused to a C-terminal domain (PgVPolX), which shows homology with NAD+-dependent DNA ligases. Analysis of the biochemical features of the purified enzyme revealed that PgVPolX is a multifunctional protein that could act as a “Swiss army knife” enzyme during BER since it is endowed with: 1) a template-directed DNA polymerization activity, preferentially acting on DNA structures containing gaps; 2) 5′-deoxyribose-5-phosphate (dRP) and abasic (AP) site lyase activities; and 3) an NAD+-dependent DNA ligase activity. We show how the three activities act in concert to efficiently repair BER intermediates, leading us to suggest that PgVPolX may constitute, together with the viral AP-endonuclease, a BER pathway. This is the first time that this type of protein fusion has been demonstrated to be functional

Efficient computation of three-dimensional flow in helically corrugated hoses including swirl

In this article we propose an efficient method to compute the friction factor of helically corrugated hoses carrying flow at high Reynolds numbers. A comparison between computations of several turbulence models is made with experimental results for corrugation sizes that fall outside the range of validity of the Moody diagram. To do this efficiently we implement quasi-periodicity. Using the appropriate boundary conditions and matching body force, we only need to simulate a single period of the corrugation to find the friction factor for fully developed flow. A second technique is introduced by the construction of an appropriately twisted wedge, which allows us to furthermore reduce the problem by a further dimension while accounting for the Beltrami symmetry that is present in the full three-dimensional problem. We make a detailed analysis of the accuracy and timesaving that this novelty introduces. We show that the swirl inside the flow, which is introduced by the helical boundary, has a positive effect on the friction factor. Furthermore, we give a prediction for which orrugation angles the assumption of axisymmetry is no longer valid. It then has to make place for Beltrami-symmetry if accurate results are required

Fingering Instability in Combustion

A thin solid (e.g., paper), burning against an oxidizing wind, develops a fingering instability with two decoupled length scales. The spacing between fingers is determined by the P\'eclet number (ratio between advection and diffusion). The finger width is determined by the degree two dimensionality. Dense fingers develop by recurrent tip splitting. The effect is observed when vertical mass transport (due to gravity) is suppressed. The experimental results quantitatively verify a model based on diffusion limited transport

Krabbe disease successfully treated via monotherapy of intrathecal gene therapy

Globoid cell leukodystrophy (GLD; Krabbe disease) is a progressive, incurable neurodegenerative disease caused by deficient activity of the hydrolytic enzyme galactosylceramidase (GALC). The ensuing cytotoxic accumulation of psychosine results in diffuse central and peripheral nervous system (CNS, PNS) demyelination. Presymptomatic hematopoietic stem cell transplantation (HSCT) is the only treatment for infantile-onset GLD; however, clinical outcomes of HSCT recipients often remain poor, and procedure-related morbidity is high. There are no effective therapies for symptomatic patients. Herein, we demonstrate in the naturally occurring canine model of GLD that presymptomatic monotherapy with intrathecal AAV9 encoding canine GALC administered into the cisterna magna increased GALC enzyme activity, normalized psychosine concentration, improved myelination, and attenuated inflammation in both the CNS and PNS. Moreover, AAV-mediated therapy successfully prevented clinical neurological dysfunction, allowing treated dogs to live beyond 2.5 years of age, more than 7 times longer than untreated dogs. Furthermore, we found that a 5-fold lower dose resulted in an attenuated form of disease, indicating that sufficient dosing is critical. Finally, postsymptomatic therapy with high-dose AAV9 also significantly extended lifespan, signifying a treatment option for patients for whom HSCT is not applicable. If translatable to patients, these findings would improve the outcomes of patients treated either pre- or postsymptomatically

FGFR2 signaling underlies p63 oncogenic function in squamous cell carcinoma

Oncogenic transcription factors drive many human cancers, yet identifying and therapeutically targeting the resulting deregulated pathways has proven difficult. Squamous cell carcinoma (SCC) is a common and lethal human cancer, and relatively little progress has been made in improving outcomes for SCC due to a poor understanding of its underlying molecular pathogenesis. While SCCs typically lack somatic oncogene-activating mutations, they exhibit frequent overexpression of the p53-related transcription factor p63. We developed an in vivo murine tumor model to investigate the function and key transcriptional programs of p63 in SCC. Here, we show that established SCCs are exquisitely dependent on p63, as acute genetic ablation of p63 in advanced, invasive SCC induced rapid and dramatic apoptosis and tumor regression. In vivo genome-wide gene expression analysis identified a tumor-survival program involving p63-regulated FGFR2 signaling that was activated by ligand emanating from abundant tumor-associated stroma. Correspondingly, we demonstrate the therapeutic efficacy of extinguishing this signaling axis in endogenous SCCs using the clinical FGFR2 inhibitor AZD4547. Collectively, these results reveal an unanticipated role for p63-driven paracrine FGFR2 signaling as an addicting pathway in human cancer and suggest a new approach for the treatment of SCC